Processing, Cryopreserving, and Controlling the Quality of HSC

AbstractThe efficiency of an autologous, as well as an allogeneic, HSC graft is mainly determined by the number of CD34+ cells present. The dose of transplanted CD34+ cells per kg body weight (bw) determines the kinetics of the neutrophil and platelet engraftment after auto-HCT (Weaver et al. Blood 86:3961–9, 1995). The measurement of CD34+ cells by flow cytometry is, therefore, an important method to assess the graft quantity and quality.