FIGURE 4 from Inhibition of NEK2 Promotes Chemosensitivity and Reduces KSHV-positive Primary Effusion Lymphoma Burden

JH295 treatment results in G₁ stage cell-cycle arrest in PEL. A, Cell-cycle analysis via PI staining and flow cytometry of PEL cells treated with or without JH295. Data represent mean ± SD of three independent biological replicates and were analyzed for the G₁ cell population using two-way ANOVA with Dunnett multiple comparisons. ****, P < 0.0001. BC1 24 hours *, P = 0.0172; JSC1 24 hours **, P = 0.0039. B, Quantification of the sub-G₁ population in PEL cells treated with or without JH295. Data are from the same experiments as A, are plotted as individual data points representing mean ± SEM, and analyzed using two-way ANOVA with Dunnett multiple comparisons. Data are from three independent biological replicates, each performed in duplicate except BCBL1, which was performed in duplicate once and in single replicates twice. ****, P < 0.0001. BCBL1 ***, P = 0.0003; BC1 24 hours **, P = 0.0061.