Simple data-driven agent-based model of scratch assays on healthy and keloid fibroblasts

In this study we propose a novel agent-based model to reproduce and propose new hypotheses on the biological mechanisms of cell-cell interactions and cell migration from data obtained during scratch assay with healthy and keloid fibroblasts. The advantage of the agent-based model we propose in this paper lies in its simplicity: only three governing parameters. We conducted a parametric sensitivity analysis and we incorporated the evaluation of contact inhibition of locomotion, aligning with the observed loss during malignant invasion. To study invasion modalities, we conducted in vitro wound healing assays using healthy and pseudo-tumoral (keloid) fibroblasts under diverse conditions: control, macrophage type 1 secretome, and macrophage type 2 secretome. Mitomycin inhibition of proliferation isolated the contribution of migration to wound filling. Our agent-based mathematical model describes configurations based on our microscopy imaging and statistical data, which enables quantitative comparisons between our experimental and numerical results. Calibration and evaluation were performed on the same experiments, enriched by external datasets. With only three governing parameters, our model not only demonstrated good agreement (8.78% to 18.75% error) with external evaluation datasets for all experimental configurations but also provided us with a nuanced understanding of keloid fibroblast behavior during wound healing, especially regarding contact inhibition dynamics. ### Competing Interest Statement The authors have declared no competing interest.