Role of Chemotaxis of V2 T Cells to the Synovium in the Pathogenesis of Acute Gouty Arthritis

Background: This study aimed to explore the potential roles of V delta 2 T cells in the pathogenesis of acute gouty arthritis. Methods: Patients with gout (n=86), rheumatoid arthritis (n=7), osteoarthritis (n=9), and healthy controls (n=40) were enrolled. gamma 8T cell frequency, chemokine receptor expression and production of pro -inflammatory cytokines in cells from peripheral blood and synovial fluid samples were quantified by flow cytometry. The chemotaxis ability of V delta 2 T cells was determined by transwell migration assay. The levels of chemokines were also detected by enzyme -linked immunosorbent assay. Results: Peripheral V delta 2 T cells had significantly lower frequencies in acute gout patients than that in healthy controls (P<0.001). These peripheral V delta 2 T cells were negatively correlated with inflammatory markers. V delta 2 T cells from acute gout patients accumulated in synovial fluid, as evidenced by a higher abundance of V delta 2 T cells in it than that in the serum (P<0.01). And V delta 2 T cells expressed a high level of C -X-C chemokine receptor 3 (P=0.035), and its corresponding chemokine C -X-C motif chemokine ligand 10 showed a high concentration in synovial fluid (P<0.05). V delta 2 T cells from synovial fluid of acute gout patients produced a high level of interleukin-17 (P=0.033). Conclusion: The up -regulated expression of C -X-C chemokine receptor 3 on V delta 2 T cells potentially facilitates their infiltration into synovial fluid during acute gouty arthritis. Further production of interleukin-17 by V delta 2 T cells may contribute to the pathogenesis of This sheds new on novel V delta 2 T cells -based for treatment.