Resveratrol-loaded sulfated Hericium erinaceus p-glucan-chitosan nanoparticles: Preparation, characterization and synergistic anti-inflammatory effects

Resveratrol (RES) is a natural polyphenol with excellent biological activity. But the poor stability and bioavailability of RES severely limit its application. Thus, the resveratrol-loaded sulfated Hericium erinaceus p-glucan-chitosan nanoparticles (DS-CS-RES NPs) were prepared using electrostatic self-assembly to solve these problems in this study. The structure of DS-CS-RES NPs was spherical or sub spherical shape with small average particle size (191.07 nm), which was characterized by FT-IR, FS, XRD and TEM. DS-CS-RES NPs exhibited good stability and RES had a sustainable release from the nanoparticles in gastrointestinal digestion. Meanwhile, DSCS-RES NPs could improve the inflammatory injury of LPS stimulated RAW264.7 macrophages by inhibiting the production of NO, IL -1p, IL -6 and TNF-a. Furthermore, DS-CS-RES NPs had strong anti-inflammatory activity by regulating protein levels of NF-xB p65, STAT1 and TLR4 through NF-xB and JAK-STAT1 signaling pathway in vitro, and sulfated H. erinaceus p-glucan-chitosan nanoparticle (DS-CS NPs) and RES had synergistic antiinflammatory effect. Overall, DS-CS NPs can serve as a potential green and safe functional carrier for encapsulating resveratrol, which can improve its anti-inflammatory activity. This work may be conducive to the development of functional carrier for encapsulating RES and applications of hydrophobic active molecules in functional foods or medicines.