Anti-inflammatory potential of Pajanelia longifolia (Willd.) K. Schum. ethanolic leaf extract

Pajanelia longifolia (Willd.) K. Schum. (P. longifolia) commonly known as Pajanelia or tender wild jack has been used as traditional herbal medicine in India for the treatment of a variety of ailments such as arthritis, stomach disorders, wound healing, skin diseases and obesity. Inflammation is a part of the vascular tissue's complicated biological reaction to adverse stimuli like pathogens, damaged cells, or irritants. Inflammation is a common physiological response to various stimuli to limit damage and facilitate tissue healing. The present investigation aimed to determine the effectiveness of P. longifolia in inflammation in rats by the carrageenan-induced rat paw oedema method, and to assess the protection of mast cell degranulation by the in vitro method. In the carrageenan-induced rat paw oedema model, 2% carrageenan was used for inducing the disease and diclofenac sodium (20 mg/kg) was used as the standard. In the mast cell degranulation method, propranolol was used as the degranulating substance and results were compared for each treatment group. The anti-inflammatory activities were studied by using a digital plethysmometer for measuring paw oedema. Dose-dependent inhibition of propranolol-induced degranulation of mast cells was observed. The finding of the study reveals that P. longifolia exhibits dose -dependent anti-inflammatory activity. Molecular docking analysis confirmed its effect on preventing inflammation.