FIGURE 1 from Identification of Enhanced Vaccine Mimotopes for the p15E Murine Cancer Antigen

The wild-type p15E short peptide is poorly immunogenic and does not inhibit tumor growth. C57BL/6 mice were vaccinated intramuscularly with 2 μg of E7 or p15E peptide displayed on CPQ liposomes (along with 8 μg CoPoP, 3.2 μg PHAD and 3.2 μg QS-21) on day 0 and 7; then blood was collected for Ag-specific CD8⁺ T-cell tetramer staining on day 14 and E7 vaccinated mice were challenged with 1 × 10⁵ TC-1 cells and p15E vaccinated mice were challenged with 1 × 10⁶ MC38 cells. (A) Ag-specific CD8⁺ T-cell percentages in CD8⁺ T cells in blood. TC-1 (B) and MC38 (C) tumor volume in non-vaccinated control (Ctrl) mice, or mice immunized with the E7 or p15E (wild-type) peptides. Statistical analysis shows unpaired t test. ****, P < 0.0001. Error bars show mean + SD for 3 mice per group.