FIGURE 3 from Identification of Enhanced Vaccine Mimotopes for the p15E Murine Cancer Antigen

The p15E-3M mimotope induces improved cytotoxic T-cell responses in vitro. A, Mean fluorescence intensity (MFI) of the indicated peptides bound to RMA-S cells after staining with PE-labeled anti-mouse H-2K^b antibody. B, Binding stability of the indicated peptide with RMA-S MHC-I molecules. Mice were immunized with 2 µg of the indicated peptide liposomal vaccine per injection on days 0 and day 7. C, The percentage of p15E-specific CD8⁺ T cells in all CD8⁺ T cells from blood tested on day 14. Splenocytes were collected on day 14 for cytotoxic CD8⁺ T-cell generation and lytic activity evaluation from the indicated groups (D). Splenocytes were stimulated with the different peptides as indicated. E, IFNγ ELISPOT assays were recorded. Datapoints represent mean values of 3 mice from the same group for A, B, and E; represent mean values of four mice for D. Datapoints represent individual values for each mouse for C. Statistical analysis applied one-way ANOVA followed by Tukey multiple comparisons tests. *, **, ***, and **** indicating P < 0.05, 0.01, 0.001, and 0.0001, or respectively compared with the 3M group in E; ^#,^### indicating P < 0.05, 0.001, respectively compared with 3C group in E. Error bars show mean +, ±, or − SD.