Supplementary Figure S1 from XTX301, a Tumor-Activated Interleukin-12 Has the Potential to Widen the Therapeutic Index of IL12 Treatment for Solid Tumors as Evidenced by Preclinical Studies

Supplemental Figure S1: A representative triplex fluorescent western blot image showing cleavage of XTX301 in primary human tumor specimens. Antibodies against human IL-12 (Green), human IL-12Rβ2 (Red) and human IgG (Blue) were used, a merged image is shown in the figure. The 145 Kda band is the intact XTX301 or non-cleavable construct, the 85Kda band is the cleaved XTX301/unmasked construct, the green arrow indicates free IL-12 released upon cleavage of XTX301. The % of cleavage mXT301 refers to the percentage of cleaved molecule compared to the total (intact and cleaved) molecule detected by western blot using the IL-12R2 signal.