Harzianic acids and oxazolidinone from the endophytic fungus Ilyonectria sp. and their cytotoxicity activity

Four undescribed compounds including three harzianic acids ( 1 , 3 and 4 ) and one oxazolidinone ( 2 ), along with three known ones ( 5 - 7 ) were isolated from the solid fermented product of endophytic fungus Ilyonectria sp., their structures were elucidated as 1-amino-harzianic acid ( 1 ), ilyonectria-oxazolidinone ( 2 ),10 ' -nor- isoharzianic acid ( 3 ), isohomoharzianic acid ( 4 ), harzianic acid ( 5 ), isoharzianic acid ( 6 ), homoharzianic acid ( 7 ) by means of detailed chemical evidences and spectroscopic data analysis. All the compounds were evaluated for cytotoxicity against SMMC-7721 human cancer cell lines by MTS assay. Among the seven tested compounds, 1-amino-harzianic acid ( 1 ) demonstrated well cytotoxic activity against SMMC-7721 with IC 50 value of 26.84 mu M. The results of molecular docking indicated that compound exhibited moderate anti -tumor activity may through binding to apoptosis related proteins.