Potential-resolved electrochemiluminescence for simultaneous determination of multiplex bladder cancer markers

A novel ECL immunosensor was developed for simultaneous determination of multiplex bladder cancer markers. DNA tetrahedra act as capture probes, while Ru-MOF@AuNPs and AuAgNCs act as signal reporters, yielding well-separated signals reflecting NUMA1 and CFHR1 concentrations. This strategy offers a new platform for clinical immunoassays, enabling simultaneous multiplex tumor marker detection. A novel ECL immunosensor was developed for detecting multiple markers. It utilizes DNA tetrahedra as capture probes, Ru-MOF@AuNPs and AuAgNCs as potential-resolved signal reporters, providing distinct signals for NUMA1 and CFHR1 concentrations.