Implementable Assay for Monitoring Minimum Residual Disease after Radical Treatment for Colorectal Cancer.
Cancer science(2024)
摘要
Considering the cost and invasiveness of monitoring postoperative minimal residual disease (MRD) of colorectal cancer (CRC) after adjuvant chemoradiotherapy (ACT), we developed a favorable approach based on methylated circulating tumor DNA to detect MRD after radical resection. Analyzing the public database, we identified the methylated promoter regions of the genes FGD5, GPC6, and MSC. Using digital polymerase chain reaction (dPCR), we termed the "amplicon of methylated sites using a specific enzyme" assay as "AMUSE." We examined 180 and 114 pre- and postoperative serial plasma samples from 28 recurrent and 19 recurrence-free pathological stage III CRC patients, respectively. The results showed 22 AMUSE-positive of 28 recurrent patients (sensitivity, 78.6%) and 17 AMUSE-negative of 19 recurrence-free patients (specificity, 89.5%). AMUSE predicted recurrence 208 days before conventional diagnosis using radiological imaging. Regarding ACT evaluation by the reactive response, 19 AMUSE-positive patients during their second or third blood samples showed a significantly poorer prognosis than the other patients (p = 9E-04). The AMUSE assay stratified four groups by the altered patterns of tumor burden postoperatively. Interestingly, only 34.8% of cases tested AMUSE-negative during ACT treatment, indicating eligibility for ACT. The AMUSE assay addresses the clinical need for accurate MRD monitoring with universal applicability, minimal invasiveness, and cost-effectiveness, thereby enabling the timely detection of recurrences. This assay can effectively evaluate the efficacy of ACT in patients with stage III CRC following curative resection. Our study strongly recommends reevaluating the clinical application of ACT using the AMUSE assay. We developed a method called AMUSE, utilizing methylated circulating tumor DNA, to detect minimal residual disease (MRD) in colorectal cancer (CRC) patients after treatment. The AMUSE assay displayed high sensitivity and specificity in predicting cancer recurrence earlier than traditional imaging methods and also evaluated the efficacy of adjuvant chemoradiotherapy in these patients. The study highlights the potential of the AMUSE assay for noninvasive, cost-effective MRD monitoring and suggests reconsidering chemoradiotherapy application using this method.image
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关键词
DNA methylation,drug resistance,gene expression analysis,tumor biomarker
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