Gene knock-out in Mycobacterium abscessus using Streptococcus thermophilus CRISPR/Cas

Suriya Akter,Elisabeth Kamal,Carsten Schwarz,Astrid Lewin

Journal of Microbiological Methods（2024）

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摘要

The CRISPRi system using dCas9Sth1 from Streptococcus thermophilus developed for Mycobacterium tuberculosis and M. smegmatis was modified to allow gene knock-out in M. abscessus. Efficacy of the knock-out system was evaluated by applying deletions and insertions to the mps1 gene. A comparative genomic analysis of mutants and wild type validated the target specificity.

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关键词

CRISPR/CAS,Gene knock-out,mps1,Mutagenesis,Mycobacterium abscessus,Nontuberculous mycobacteria

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