Bimodal analysis of intracellular in situ self-assembled Au complexes based on single-cell electrochemistry

Gold complexes have shown great potential for clinical applications in cancer chemotherapy. However, the unclear mechanisms of tumor heterogeneity and induced drug resistance hinder the effectiveness of gold complex therapy. To address this issue, single-cell electrochemical detection strategies have been widely reported for bioimaging and bioanalysis, due to their in-situ, real-time, and high-resolution characteristics. In this study, the self-assembled process of gold complexes was investigated by detecting hydrogen peroxide release at the singlecell level using nanoelectrodes and scanning electrochemical microscopy. This detection strategy could provide more spatio-temporal information on the synthesis of bioresponsive fluorescent nanoprobes.