Establishment of HPLC method and optimization of ultrasonic-assisted extraction process for two lignan active substances in Asarum heterotropoides

In the present study, developed a dependable HPLC technique to quantify asarinin and sesamin in Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. The optimal conditions for the ultrasonic -assisted extraction were determined by conducting single -factor experiment, Plackett-Burman design experiment, and Box-Behnken response surface method. Based on actual operation factors and analysis, the optimal extraction parameters were methanol volume fraction of 80% (v/v), liquid -to -material ratio of 10:1 (mL/g), ultrasonic time of 40 min, ultrasonic temperature of 51 degrees C, and ultrasonic power of 300 W. When subjected to these conditions, the average asarinin and sesamin content were found to be 13.40 mg/g and 2.39 mg/g, respectively, with a relative error of 1.04% and 3.46%, which was consistent with the theoretical value. This study can offer a theoretical reference for the extraction of asarinin and sesamin in Asarum.