Comparative analysis of the diversity of symbionts in fat body of long- and short-winged brown planthoppers

The microbial community structure plays an important role in the internal environment of brown planthopper (BPH), Nilaparvata lugens (Hemiptera: Delphacidae), which is an indispensable part to reflect the internal environment of BPH. Wing dimorphism is a strategy for balancing flight and reproduction of insects. Here, quantitative fluorescence PCR was used to analyse the number and changes of the symbionts in the fat body of long- and short-winged BPHs at different developmental stages. A metagenomic library was constructed based on the 16 S rRNA sequence and internal transcribed spacer sequence for high-throughput sequencing, to analyze the community structure and population number of the symbionts of long- and short-winged BPHs, and to make functional prediction. This study enriches the connotation of BPH symbionts, and laid a theoretical foundation for the subsequent study of BPH-symbionts interaction and the function of symbionts in the host. Sequencing analysis was conducted on the symbiotic microorganisms within the fat body of different wing morphs of brown planthoppers. This study investigated the abundance, species diversity, community structure, and functional predictions of symbiotic fungi and bacteria, revealing the impact of wing morphs on symbiotic microorganisms. image The symbiotic bacterial populations within the fat bodies of brown planthopper (BPHs) with wing dimorphism significantly after eclosion. The species richness and species composition uniformity of fat body fungi in long wing BPH were higher than those in short wing BPH, and the opposite was true for bacteria. Different winged BPHs have variations in dominant symbiotic bacteria within their fat bodies. Prediction and analysis of symbiotic bacterial and fungal communities function of different winged BPHs.