Protocol for Generating Customizable and Reproducible Plots of Sequencing Coverage Data Using the Seqndisplayr Package
STAR protocols(2024)
摘要
The widespread usage of next-generation sequencing methods for functional genomics studies requires standardized tools for consistent visualization of the associated data. Here, we present seqNdisplayR, an R package for plotting standard sequencing data coverage within a genomic region of interest in a customizable and reproducible manner. We describe steps for installing software, preparing data files, choosing options, and plotting data. This tool is readily available for users with no prior experience with R through the “Shiny app” interface.For complete details on the use and execution of this protocol, please refer to Lykke-Andersen et al.,1 Gockert et al.,2 and Rouviere et al.3
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关键词
Sequencing,RNA-seq,ChIP-seq,Gene Expression,Chromatin immunoprecipitation,ChIP
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