FIGURE 5 from Nanoparticle-mediated Photodynamic Therapy as a Method to Ablate Oral Cavity Squamous Cell Carcinoma in Preclinical Models

Antitumor treatment response to PS-PDT (10 mg/kg, 24 hour DLI, 100 J/cm²) in tumor-bearing mice models of oral cavity cancer. Representative posttreatment images in subcutaneous Cal-33 tumors (A) and in subcutaneous MOC22 tumors (B) following treatment on day 0. C, Fold change in tumor volume in subcutaneous Cal-33 tumors following treatment on day 0. N = 20 tumors (PS-PDT), 17 tumors (untreated control), nine tumors (drug control), and seven tumors (light control). D, Fold change in tumor volume in subcutaneous MOC22 tumors following treatment on day 0. N = 11 tumors (PS-PDT), and 13 tumors (untreated control). For C and D, Unitless. Mean + SD. Day 14 statistics: multiple unpaired (two-sample) t tests using Holm-Šídák correction method for multiple comparisons and ⍺ = 0.05. Additional PS-PDT efficacy data and statistical analysis are provided in Supplementary Tables S9 and S10. E, Hematologic changes in immune competent MOC22 tumor-bearing mice 24 hours post-PS administration (“Post-PS”) and 72 hours post-PS-PDT treatment (“Post-PDT”). Units given. Bar plot with mean + SD. ● represent individual replicates. Normal limits for each parameter represented by dotted lines. N = 10 Post-PS mice and 8 Post-PDT mice. Statistics: unpaired t tests without correction for multiple comparisons and ⍺ = 0.05. Additional hematology and blood chemistry analysis is provided in Supplementary Fig. S7. RBC, red blood cells (× 10⁶ cells/µL); HCT, hematocrit (%); PLT, platelets (× 10³/µL); WBC, white blood cells (× 10³ cells/µL); NEUT, neutrophil count (× 10³ cells/µL); NEUT%, relative neutrophil count (%).