Search for Metabolomic Markers of Hypertensive Conditions of Different Genesis: Experimental Study

A personalized approach to the diagnosis and treatment of arterial hypertension requires a comprehensive analysis of the pathogenetic mechanisms underlying the disease. To determine specific metabolomic markers of various hypertensive conditions, four groups of experimental animals were studied: WAG rats (normotensive control); ISIAH rats with inherited stress-induced arterial hypertension (AH); L-NAME-treated rats with hypertension induced by endothelial dysfunction; rats with hypertension caused by DOCA administration in combination with the salt loading. Rat blood serum samples were analyzed by NMR spectroscopy. The metabolomic analysis differentiated the hypertensive conditions of various origins using group-specific blood serum metabolomic biomarkers. Rats with DOCA-salt hypertension are characterized by increased concentration of choline. Hypertension associated with endothelial dysfunction induced by L-NAME administration was accompanied by a decrease in the levels of tyrosine, serine and glycine. Distinctive features of ISIAH rats are increased concentrations of ornithine (urea and nitric oxide cycle), valine, leucine, isoleucine, myo-inositol, glutamate, glutamine (glucose metabolism).