Non-photochemical quenching upregulation improves water use efficiency and reduces whole plant level water consumption under drought.

For crop production, the water supply limitations will likely become a bigger restriction underlining a need for crops that use less water per mass of production. Therefore, water use efficiency becomes a constraint in obtaining resilient and productive crops. We hypothesized that under drought conditions via modulation of chloroplast signal for stomatal opening by upregulation of non-photochemical quenching (NPQ) it is possible to improve water use efficiency. Nicotiana tabacum plants with strong overexpression of photosystem II subunit S (PsbS), a key protein to NPQ, were grown under varied levels of drought. The PsbS-overexpressing lines lost 11% less water per CO2 fixed under drought which did not have a significant effect on plant size. Depending on growth conditions PsbS-overexpressing lines on the whole plant level consumed from 4% to 30% less water than the corresponding wildtype. The leaf water and chlorophyll contents showed a positive relation with the level of NPQ. Our study provides proof of concept and as such is an important step towards engineering crops with improved water use efficiency.