Novel class of peptides disintegrating biological membranes to aid in the characterization of membrane proteins
Journal of Biological Chemistry(2024)
Abstract
Styrene-maleic acid (SMA) and similar amphiphilic copolymers are known to cut biological membranes into lipid nanoparticles/nanodiscs containing membrane proteins apparently in their relatively native membrane lipid environment. Our previous work demonstrated that membrane raft microdomains resist such disintegration by SMA. The use of SMA in studying membrane proteins is limited by its heterogeneity and the inability to prepare defined derivatives. In the present paper, we demonstrate that some amphiphilic peptides structurally mimicking SMA also similarly disintegrate cell membranes. In contrast to the previously used copolymers, the simple peptides are structurally homogeneous. We found that their membrane disintegrating activity increases with their length (reaching optimum at 24 amino acids) and requires a basic primary structure, i.e. (XXD)n, where X represents a hydrophobic amino acid (optimally phenylalanine), D aspartic acid and n is the number of repeats of these triplets. These peptides may provide opportunities for various well-defined potentially useful modifications in the study of membrane protein biochemistry. Our present results confirm a specific character of membrane raft microdomains.
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Key words
Peptides,lipid raft,membrane,membrane proteins,lymphocyte,leukocyte
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