Effects of Hydrolyzed Collagen as a Dietary Supplement on Fibroblast Activation: A Systematic Review

Purpose Our objective was to conduct a systematic review of the effects of hydrolyzed collagen supplementation on the proliferation and activation of fibroblasts. Methods The search was conducted for journals that published articles in the English language, peer-reviewed, meeting the following criteria: a) randomized clinical trials, b) randomized studies in animals or humans, c) in vitro studies, d) studies using hydrolyzed collagens or collagen peptides, d) studies assessing alterations on fibroblasts as primary or secondary outcome. We utilized the main journal databases PUBMED/WEB OF SCIENCE and ongoing reviews by PROSPERO. For bias risk and methodological quality, we used an adaptation of the Downs and Black checklist. Our review followed the PRISMA checklist, conducted from February 2024 to the first week of March 2024, by two independent researchers (P.A.Q.I. and R.P.V.). Results Eleven studies were included in this review, where our findings reinforce the notion that hydrolyzed collagens or collagen peptides at concentrations of 50-500 μg/ml are sufficient to stimulate fibroblasts in human and animal tissues without inducing toxicity. Different enzymatic processes may confer distinct biological properties to collagens, allowing for scenarios favoring fibroblast promotion or antioxidant effects. Lastly, collagens with lower molecular weights exhibit greater bioavailability to adjacent tissues. Conclusion Hydrolyzed collagens or collagen peptides with molecular sizes ranging from <3 - 3000 KDa promote the stimulation of fibroblasts in human tissues. ### Competing Interest Statement The authors have declared no competing interest.