Omics Mass Spectrometry Analysis of Canine Plasma

Abstract: Blood derived products, such as autologous plasma, have high clinical importance and are applied in numerous therapeutic fields. The preparation of autologous plasma from the patient's own blood is easy to perform by centrifugation, however, the prep-aration procedure can significantly affect the blood cells, platelets and vesicles in the sample. Therefore, it is of utmost importance to understand the impact of sample pro-cessing on the chemical composition of plasma preparations as well as on their biolog-ical activity. Here, we present a mass spectrometry-based plasma profiling method in which three compound groups: lipids, proteins and glycoproteins are analysed in a single workflow. Analysis of the chemical composition of plasma samples prepared by different centrifugation protocols revealed differences in the lipid and glycoprotein profiles, demonstrating the importance of standardized protocols for the preparation of plasma products. Keywords: Plasma; Lipids; Proteins; Glycoproteins; Mass Spectrometry, Ion Mobility