Biomass of Euglena gracilis Produced in Mineral Medium Supplemented with Craft Brewery Residue

Giséle Alves,Tiele Medianeira Rizzetti, Maria Angelica Oliveira Linton,Michele Hoeltz,Adriana Dupont, Vitória Costa da Rocha, Leonardo Ritzel,Rosana de Cassia Souza Schneider

Waste and Biomass Valorization(2024)

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Abstract
Industrial waste from craft breweries can be used to grow microorganisms due to its high nutrient content. Reusing waste and reducing its improper disposal are great economic and environmental strategies. Thus, the objective was to cultivate Euglena gracilis in benchtop 1-L photobioreactors using different concentrations of NPK-enriched (0.5–6.0 g L− 1) mineral medium with and without the supplementation with a mixture of brewing residues (BR-Mix) with increase of scale (30 L) and BR-Mix supplementation (30 Statement of Novelty The clean industrial residues collected from craft breweries present an opportunity for cultivating microorganisms, including the microalgae genus Euglena, an interesting microalgal biomass. This potential not only addresses waste management issues but also contributes to economic and environmental sustainability by reducing improper disposal. We examined the influence of the brewery waste mixture on enhancing the Euglena gracilis growth potential. The subsequent phase of our study involved scaling up the process to a 30 L photobioreactor. Throughout our investigation, we analyzed parameters such as carbohydrates, lipids, proteins, and antioxidant potential under these conditions with a mineral medium and brewery waste.
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