Development of a Matrix Certified Reference Material for Quantification of Aflatoxin B₁ in Peanut Oils

Aflatoxin B-1 in a peanut oil matrix certified reference material (CRM, GBW10176) has been issued for accurate determination of aflatoxin B-1 in real samples. This matrix CRM was prepared from commercial peanut oils naturally contaminated by aflatoxin B-1, with the aid of a liquid-liquid extraction process. Specially, the certified value of CRM was determined using two individual approaches, liquid chromatography-isotope dilution tandem mass spectrometry and standard addition liquid chromatography-fluorescence detection techniques. More importantly, assessments of homogeneity and short- and long-term stabilities confirmed that the GBW10176 CRM was sufficiently homogeneous between packages or within package, and it could be stable up to 7 days stored below 50 degrees C or 18 months at 4 degrees C. The metrological traceability of GBW10176 was ensured by the application of pure aflatoxin B-1 (GBW10172). Its uncertainty was evaluated with the contributions from the characterization, homogeneity, and stability. Resultantly, the certified value of the aflatoxin B-1 CRM (GBW10176) was 23.4 mu g/kg with an uncertainty of 3.1 mu g/kg (k = 2).