Unlocking the potential of one-carbon gases (CO₂, CO) for concomitant bioproduction of -carotene and lipids

This study investigates the use of a Yarrowia lipolytica strain for the bioconversion of syngas-derived acetic acid into beta-carotene and lipids. A two-stage process was employed, starting with the acetogenic fermentation of syngas by Clostridium aceticum, metabolising CO, CO2, H-2, to produce acetic acid, which is then utilized by Y. lipolytica for simultaneous lipid and beta-carotene synthesis. The research demonstrates that acetic acid concentration plays a pivotal role in modulating lipid profiles and enhancing beta-carotene production, with increased acetic acid consumption leading to higher yields of these compounds. This approach showcases the potential of using one-carbon gases as substrates in bioprocesses for generating valuable bioproducts, providing a sustainable and cost-effective alternative to more conventional feedstocks and substrates, such as sugars.