Host specific Eimeria genus diagnosis and qPCR development in Ovis aries and Capra hircus

JOURNAL OF MICROBIOLOGICAL METHODS(2024)

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摘要
The objective of the present study was to develop a real-time PCR (qPCR) technique for the diagnosis of Eimeria spp. in Ovis aries and Capra hircus. The qPCR technique was developed using SYBR Green, resulting in a PCR with high sensitivity, specificity, and reproducibility.
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关键词
18S rRNA,Ovis aries,Capra hircus,Coccidiosis,Eimeria,qPCR
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