Wide detection range for non-enzymatic glucose monitoring by utilizing LDHs-coated CuO nanowires in biological media

Inorganic nanostructured materials show significant potential in improving diabetes treatments by serving as glucose-sensing elements that eliminate the requirement for expensive enzymatic agents. However, most nonenzymatic sensors suffer from limited selectivity and reliability, which compromise their suitability for practical applications to body fluids. In this study, we developed a novel non-enzymatic glucose sensor that exhibits a remarkable selectivity and high detection range (<8 mM) in real human serum. The sensor operated effectively across a wide range of glucose concentrations, with the ability to detect glucose levels of up to 8 mM in human serum. Critically, the sensor demonstrated a significant resistance to interference from other substances, which is a common challenge in biological sensing. Rigorous testing confirmed the precision of the sensor, as evidenced by its impressively low relative standard deviation (RSD) of less than 1.56 %, thereby highlighting its consistent reliability for potential clinical applications. Overall, the CuO nanowires (NWs)-NiFe layered double hydroxides (LDHs) sensor is significantly promising as a reliable, highly reversible, and cost-effective solution for glucose detection and continuous glucose monitoring systems.