Dermal absorption of high molecular weight parent and alkylated polycyclic aromatic hydrocarbons from Manufactured Gas Plant soils using in vitro assessment

An enhanced in vitro human dermal bioavailability method was developed to measure the release of 20 parent and 7 alkylated high molecular weight (HMW) polycyclic aromatic hydrocarbons (PAHs) from contaminated soils collected from five former manufactured Gas Plants (MGP) in England. GC-MS/MS was used to quantify HMW PAHs in soil, Strat-M artificial membrane representing skin, and synthetic receptor solution (RS) representing systemic circulation at 1-h, 10-h, and 24-h timesteps. Fluoranthene and pyrene exhibited the highest fluxes from soils to membrane (ranging from 9.5 - 281ng/cm2/h) and soil to RS (更多