Prevention of Post-operative Bacterial Colonization on Mice Buccal Mucosa Using Biogenic Silver Nanoparticles-Coated Nylon Sutures

In this study, biogenic silver nanoparticles (AgNPs) deposited on nylon sutures using in situ technique were employed as an in vivo antibacterial and wound healing strategy on post-operative buccal mucosa. Biogenic AgNPs synthesized using phenolic-rich Eucalyptus camaldulensis extract, followed by coating on nylon monofilament surgical sutures. Subsequently, coated nylon sutures were sewn with a simple interrupt pattern at the wound of the buccal mucosa of five mice per group (coated and uncoated). Sutures were taken out at days 1, 3, and 7 post-surgery, and a biopsy specimen of buccal mucosa tissues was removed. In vivo effects including antibacterial activity, nitric oxide test, and histopathological changes were demonstrated in the buccal cavity of the mice model. AgNPs-coated nylon sutures revealed bacteriostatic effects with more than 90