Superoxide-producing associates from gastrointestinal bacteria: stimulation of its growth by exogenous superoxide-producing complex from raspberries

Aerobic organisms including the gut microbiota have an essential antioxidant status, as a result of which these bacteria protect organisms from various pathologies and diseases. The goal of the given investigation is (1) the isolation and purification of the isoforms of endogenous & Ocy;(-)(2)-producing associate from gastrointestinal bacteria (Lactobacillus rhamnosus, Lactobacillus acidophilus, Bifidobacterium bifidum); (2) determination of the effective concentrations of exogenous & Ocy;(-)(2) produced by a complex of NADPH-containing protein component and Fe(III) (NPC-Fe(III)) from raspberries on the growth of the gastrointestinal bacteria in a nutrient medium in vitro. Ion-exchange chromatography on cellulose DE-52 and gel filtration on Sephadex G-100 at the pH of 9.5 was used to isolate and purify the NLP-Nox isoforms. Specific maximal optical absorption spectra of the Nox isoforms were observed in a weakly opalescent aqueous solution of the NLP-Nox isoforms. The specific contents of these NLP-Nox isoforms, as well as their composition, the stationary concentration of produced & Ocy;(-)(2), and the mechanism of & Ocy;(-)(2) production were determined. The stimulating effect on the growth of these gastrointestinal bacteria in the nutrient medium of MRS broth and MRS agar in vitro under the influence of & Ocy;(-)(2), as a product of a new thermostable and acid-stable complex NPC-Fe(III) was determined. The NPC-Fe(III) complex, from raspberries was determined as well. Thus, for the first time, the isolation and purification of & Ocy;(-)(2)- producing thermostable NADPH-containing lipoprotein-NADPH oxidase (NLP-Nox) associate from gastrointestinal bacteria membranes (continuously producing & Ocy;(-)(2) under the aerobic conditions), and the stimulation of these bacteria growth by & Ocy;(-)(2) formed by the complex from raspberries were demonstrated. KEY POLICY HIGHLIGHTS 1. The & Ocy;(-)(2)-producing associate NLP-Nox was isolated and purified from the gut microbiota. 2. NLP-Nox associate produces & Ocy;(-)(2) by using a protein-bound non-free NADPH as a substrate. 3. The NPC-Fe(III) isolated from raspberries generates & Ocy;(-)(2). 4. The effective quantities of & Ocy;(-)(2) promotes the growth and development of bacteria.