Protocol to examine immune subpopulations in murine conjunctiva and lacrimal gland using flow cytometry
STAR PROTOCOLS(2024)
Abstract
Here, we present a protocol for the examination of immune cells in the murine conjunctiva and lacrimal gland using flow cytometry. We describe steps for dissection, preparation of high -quality single -cell suspensions, utilization of comprehensive staining panels, and optimization of flow cytometry voltage. We then detail procedures for compensation adjustments and the implementation of effective gating strategies. For complete details on the use and execution of this protocol, please refer to Ma et al.1
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Key words
Cell Biology,Cell isolation,Flow Cytometry,Immunology
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