Optimization of supercritical fluid extraction of bioactive compounds from Ammodaucus leucotrichus fruits by using multivariate response surface methodology

JOURNAL OF SUPERCRITICAL FLUIDS(2024)

引用 0|浏览7
暂无评分
摘要
This study explored supercritical fluid extraction (SFE) of bioactive molecules from Ammodaucus leucotrichus seeds, a little-known source of potent bioactive compounds. Response surface methodology assessed extraction parameters (pressure, temperature, and ethanol co-solvent) and their effects on global yield, total phenolic compound (TPC), total carbohydrate content (TCC), and anti-Alzheimer activity. Optimization via a BoxBehnken design identified the optimal conditions: 15 % ethanol, 300 bar, and 70 degrees C. Chemical profiling using GC-MS and UHPLC-q-tof-MS/MS detected over 41 metabolites. In addition, a biorefinery sequential extraction process was performed to obtain extracts with high neuroprotective potential. The SFE extracts exhibited modest AChE inhibition. Notably, sequential SFE and PLE extractions yielded extracts with enhanced antioxidant activity compared to separate processes. In conclusion, Ammodaucus leucotrichus presents a promising natural source of secondary metabolites. Its potential health benefits warrant further exploration, emphasizing the need for continued research.
更多
查看译文
关键词
Compressed fluid extraction,Response surface optimization,Ammodaucus leucotrichus,Acetylcholinesterase,Anti-inflammatory,Metabolomic,Biorefinery
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要