Enzyme Assays of E. coli and Bacillus sp . Treated with Fe Doped MgO Nanoparticles

The present study is based on the antioxidant enzyme activities of Super oxide dismutase (SOD), Malondialdehyde (MDA), Catalase (CAT), Glutathione peroxides (GSHPx) towards the Fe doped MgO on Gram-positive and Gram-negative bacterial pathogens. Oxygen, vital for most living organisms, acts as a precursor to reactive oxygen species (ROS) that can inflict damage on cellular components such as proteins, lipids, and nucleic acids. The generation of ROS is expected to cause membrane damage through lipid peroxidation, resulting in material leakage and affecting respiratory activity, ultimately leading to cell death. The significant increase in enzyme concentration in the bacterial strain treated with Fe doped MgO nanoparticle suggests an elevated production of free radicals in these strains. Consequently, cell wall disruption phenomenon can be observed. It is also seen that enzyme activity increases as the doping concentration of Fe doped MgO increases.