Towards personalized nicotinamide mononucleotide (NMN) supplementation: Nicotinamide adenine dinucleotide (NAD) concentration

Nicotinamide mononucleotide (NMN) is a precursor of nicotinamide adenine dinucleotide (NAD), which declines with age. Supplementation of NMN has been shown to improve blood NAD concentration. However, the optimal NMN dose remains unclear. This is a post -hoc analysis of a double -blinded clinical trial involving 80 generally healthy adults aged 40-65 years. The participants received a placebo or daily 300 mg, 600 mg, or 900 mg NMN for 60 days. Blood NAD concentration, blood biological age, homeostatic model assessment for insulin resistance, 6 -minute walk test, and 36 -item short -form survey (SF -36) were measured at baseline and after supplement. A significant dose -dependent increase in NAD concentration change (NAD Delta) was observed following NMN supplementation, with a large coefficient of variation (29.2-113.3%) within group. The increase in NAD Delta was associated with an improvement in the walking distance of 6 -minute walk test and the SF -36 score. The median effect dose of NAD Delta for the 6 -minute walk test and SF -36 score was 15.7 nmol/L (95% CI: 10.9-20.5 nmol/L) and 13.5 nmol/L (95% CI; 10.5-16.5 nmol/L), respectively. Because of the high interindividual variability of the NAD Delta after NMN supplementation, monitoring NAD concentration can provide valuable insights for tailoring personalized dosage regimens and optimizing NMN utilization.