A non-peptide-based fluorescent probe capable of sensitively visualizing asparagine endopeptidase

The non-peptide-based fluorescent probe QMC11 is capable of specifically targeting asparagine endopeptidase (AEP) and imaging cellular endogenous AEP. The motion of the probe can be restricted by AEP to activate fluorescence while keeping a low background signal. A sensitive and facilely synthesized fluorescent probe, QMC11, was designed for visualizing AEP.