Astraoleanosides E-P, oleanane-type triterpenoid saponins from the aerial parts of Astragalus membranaceus Bunge and their -glucuronidase inhibitory activity
Bioorganic chemistry(2024)
Abstract
Historically, Astragalus membranaceus Bunge has been used as a beneficial medicinal plant, particularly in the Asian traditional medical systems, for the treatment of various human diseases such as stomach ulcers, diarrhea, and respiratory issues associated with phlegm. In this study, a phytochemical characterization of the aerial parts of A. membranaceus led to the isolation of 29 oleanane-type triterpenoid saponins, including 11 new compounds named astraoleanosides E-P (6-9, 13, 14, 18-22), as well as 18 known ones. The structures of these compounds were elucidated using nuclear magnetic resonance (NMR) spectroscopy and high-resolution mass spectrometry. Among them, astraoleanoside H (9) and cloversaponin III (15) demonstrated the most potent fl-glucuronidase inhibitory activities, with IC50 values of 21.20 +/- 0.75 and 9.05 +/- 0.47 mu M, respectively, compared to the positive control D-saccharic acid 1,4-lactone (IC50 = 20.62 +/- 1.61 mu M). Enzyme kinetics studies were then conducted to investigate the type of inhibition exhibited by these active compounds. In addition, the binding mechanism, key interactions, binding stability, and dynamic behavior of protein-ligand complexes were investigated through in silico approaches, such as molecular docking and molecular dynamics simulations. These findings highlight the promising potential of triterpenoid saponins from A. membranaceus as lead compounds for fl-glucuronidase inhibitors, offering new possibilities for the development of therapeutic agents targeting various diseases where fl-glucuronidase plays a crucial role.
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Key words
Astragalus membranaceus,Astraoleanoside,Oleanane-type triterpenoid saponins,fl-Glucuronidase
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