Chaotropic Chromatography Method for Simultaneous Determination of Lamivudine, Abacavir and Dolutegravir in Pharmaceutical Formulations

In the pharmaceutical field, quality control professionals often face challenges when analyzing active substances that contain basic ionizable groups. One of the strategies available to address these issues is chaotropic chromatography. In this context, a novel chaotropic chromatography method for the simultaneous separation and determination of three antiretrovirols, Lamivudine (LMV), Abacavir (ABC) and Dolutegravir (DTG) in pharmaceuticals formulations has been developed and validated. An effective separation was achieved on Pentafluorophenyl propyl (5 F-PHP) column maintained at 40 °C. The used mobile phase, comprised of 50 mM sodium perchlorate buffer (pH 2.8 ± 0.05) containing 0.1