Biogenic nanoparticles from liquid and solid matrices: biochemical and biophysical properties of Extracellular Vesicles-enriched samples from human plasma and skeletal muscle tissue

The majority of studies on extracellular vesicles (EVs) focused on samples isolated from liquid matrices, such as cell culture media and blood, due to their accessibility. However, recent research highlights the emerging roles of EVs derived from solid tissues, including the brain, muscles and tumors. Investigating EVs from the extracellular matrix of solid tissues offers insights into their microenvironment and potential biological influences on surrounding cells. This study presents a method for comparing EV-enriched samples from solid (human skeletal muscle biopsy) and liquid (plasma) matrices, addressing technical challenges and minimizing biases in separation techniques. By employing optimized protocols and advanced analytical techniques, the study reveals differences in biochemical composition, nanomechanical properties, particle yield, size distribution, and colloidal stability between human muscle and plasma EVs. Understanding these distinctions may contribute to the development of novel diagnostic assays for muscular pathologies and shed light on EVs’ roles in diverse tissue environments. ![Figure][1] Graphical abstract Extracellular vesicles (EVs) studies have primarily focused on liquid matrices like cell culture media and blood. However, emerging research highlights the importance of investigating EVs from solid tissues such as the brain, muscles, liver, and tumors. This study introduces a method to compare EVs-enriched samples from solid (human skeletal muscle) and liquid (plasma) matrices, addressing technical challenges and minimizing biases in separation techniques. Utilizing standardized protocols and advanced analytical methods, the research reveals differences in biochemical composition and biophysical properties between muscle and plasma EVs. Created with [BioRender.com][2] ### Competing Interest Statement The authors have declared no competing interest. [1]: pending:yes [2]: http://BioRender.com