Determination of urinary androgens in women with polycystic ovary syndrome using LC-QqQ/MS and the application of thin film solid-phase microextraction (TF-SPME)

•Development and validation of fast and robust TF-SMPE LC-MS/MS method and application for the separation and quantification of the most discriminating urine androgens in women with PCOS.•Quantitative analysis of urinary androgens in PCOS women and evaluation of their significance as specific steroid biomarkers of this complex disorder.•Determination of the panel of three urinary androgens: T+DHT+An-dione shows high predictive value for the diagnosis of PCOS (AUC of ROC curve = 0.91) than looking only for a single biomarker.