Effect of Anodic Aluminum Oxide Modified by Angelica Sinensis Polysaccharide on the Growth of Breast Cancer Cells

The surface of nanodent and nanopore structured alumina membranes with different periods were directly modified by Angelica sinensis polysaccharide (ASP) or covalently modified by ASP via.-aminopropyl triethoxysilane (KH550). After that, the morphology and cell viability of breast cancer cells MDA-MB-231 grown on the modified nanostructured surfaces were studied. It is found that nanodent and nanopore structure directly modified by ASP can effectively inhibit the viability of breast cancer cells, and have little effect on cell morphology. However, the inhibitory effect of nanodent structure on cell viability is superior to that of nanopore structure. Nanodent structure with period of 300 nm have the best inhibitory effect on cell viability, and the inhibitory rate is 27.7%. The nanodent and nanopore structures covalently modified by ASP via KH550 have a better inhibitory effect on cell viability than that directly modified ASP, and the cell morphology change obviously. A large number of lamellipodia are generated around the cell body. However, the inhibition effect of the nanodent structure on cell viability is better than that of the nanopore structure. Nanodent structure with period of 300 nm have the best inhibitory effect, and the inhibition rate is 28.2%.