Preparing Calcium Carbonate Nanoparticles from Biomass Waste and its Application as Fire-Retardant Agent

The medicinal plant is considered a double-edged sword that could be beneficial or toxic to people or animals. Accordingly, this study is concerned with the vitro and in vivo evaluation of Euphorbia schimperiana and Euphorbia balsamifera toxicity in rats. A comparison study was carried out between both extracts on THLE2 and WI38 cell lines. Furthermore, the anti-inflammatory and anti-mutagenicity influence of both target plants were studied in rats administrated monoiodoacetate. The study was supported by biochemical and histological examinations of key organs. Methanol extract of E.schimperiana and E. Balsamifera displayed IC50 values of 30.6 +/- 1.96 and 88.7 +/- 5.67 respectively on WI38 cell lines. The chloroform fraction of E. schimperiana showed the greatest safety profile with an IC50 value of 72 +/- 3.74. In contrast, the petroleum ether fraction of E. balsamifera displayed the maximum safety profile among the other fractions with an IC50 value of 56 +/- 3.74 on THLE2 cells. Male Wistar rats administered orally 2.5 mg/kg of methanol extract did not show any fatalities or ill effects. The daily dose of the two extracts demonstrated a substantial dose-dependent decrease in the percentage of MNPEs and chromosomal abnormalities; due to mono-iodoacetate administration, suggesting the valuable role of the target plants.