Preparation of W/O/W Lactiplantibacillus plantarum L₃ microcapsules using modified low methoxy pectin as wall materials and their application in simulated yoghurt fermentation systems

This work aimed to characterise water-in-oil-in-water (W/O/W) microcapsules loading Lactiplantibacillus plantarum L-3 in a simulated fermentation and storage process for yoghurt, providing support for their potential application in complex fermented milk systems. The encapsulating efficiency of microencapsulated L. plantarum L-3 with modified pectin was 96.79%, the aerobic plate count was 7.83 log cfu/g after simulated gastrointestinal treatment. The microencapsulated L. plantarum L(3 )delayed the post-acidification of MRS medium. The cell-free supernatant produced during the MRS storage period had a bacteriostatic effect. W/O/W microcapsules showed great prospects for application as a probiotics carrier in dairy products.