A potential mechanism of miana (Coleus scutellariodes) and quercetin via NF-xB in Salmonella typhi infection

Purpose: To prove the effect of Miana (M), Quercetin (Q), and the combination as an antiinflammatory agent and Cefixime (C) as an antibiotic in Balb/c mice infected with Salmonella enterica serovar Typhi (S. Typhi) and related to the dynamics of NF-xB mRNA expression and NFxB protein levels.Methods: A cohort study on male Balb/c mice with subjects consisted of 8 groups with 5 each group by administration of M, Q, M + Q, M + C, Q + C, M + Q + C, C only and sterile distilled water group as negative control. The statistical significance of the difference group was defined as P values less than 0.05.Results: Decreased mRNA expression of NF-xB, NF-xB protein levels, and bacterial load after administration of M + C, Q + C, or M + Q + C showed significant differences when compared to the negative control. The decline in NF-xB was stronger when M + Q + C was given compared to M, Q, M + Q, or C only.Conclusion: The effects of NF-xB suppression appear to be the same between the administration of M, Q and the M + Q when C added. However, the suppression of NF-xB was not significant without adding C.