Isolation and Purification of Polyhydroxyalkanoates. Scaling in Pilot Production
JOURNAL OF SIBERIAN FEDERAL UNIVERSITY-CHEMISTRY(2023)
摘要
The process of isolating and purifying poly-3-hydroxybutyrate under scaling conditions has been studied. Sodium dodecyl sulfate, sodium hypochlorite and Pemos washing powder were studied as solubilizing agents. The highest yield of 95 % and polymer purity of 99.5 % was achieved using a two- stage extraction method and Pemos powder as a solubilizing agent. As a result, it was possible to reduce the costs at the solubilization stage to 31 rubles/kg of poly-3-hydroxybutyrate. Based on the research results, a technology for the isolation and purification of polyhydroxyalkanoates was proposed.
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关键词
polyhydroxyalkanoates,poly-3-hydroxybutyrate,solubilization,extraction,bacterial biomass,extraction technology
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