Substrate-Specific Evolution of Amine Dehydrogenases for Accessing Structurally Diverse Enantiopure (R)--Amino Alcohols
ACS CATALYSIS(2024)
摘要
The biocatalytic oxidative deamination of beta-amino alcohols holds significant practical potential in kinetic resolution and/or deracemization process to access (R)-beta-amino alcohols. This study exemplifies a notable instance of acquisition and utilization of this valuable oxidative deamination activity. Initially, the mutation N261M (M0) was identified to endow a native valine dehydrogenase with oxidative deamination activity toward a few (S)-beta-amino alcohols. Subsequently, a phylogenetic analysis-guided, double-code saturation mutagenesis strategy was proposed to engineer M0's side-chain binding site. This strategy facilitated the substrate-specific evolution of M0, resulting in the creation of a panel of mutants (M1-M4) with noteworthy oxidative deamination activity toward structurally diverse (S)-beta-amino alcohols. Using these engineered amine dehydrogenases, termed as beta-amino alcohol dehydrogenases (beta-AADHs), the complete kinetic resolution and even deracemization of a range of beta-amino alcohols have been achieved. This work reports distinct biocatalysts and a synthetic strategy for the synthesis of enantiopure (R)-beta-amino alcohols and offers an innovative approach for substrate-specificity engineering of enzymes.
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关键词
biocatalysis,amine dehydrogenases,oxidativedeamination,enantiopure beta-amino alcohols,protein engineering
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