New Alkaloids and Steroids from Hydranth of Goniopora columna corals and Their Inhibiting lung cancer cell Activities

A new alkaloids, aplysingoniopora A (1), and new configuration pregnane type steroid compound, 9,17-alpha-pregn-1,4,20-en-3-one (2), and two known pregnane type steroid compounds (3 and 4) were isolated from hydranth of Goniopora columna corals. The compounds structures and absolute configurations were determined by extensive spectroscopic analysis, MS data, single-crystal X-ray diffraction analysis and quantum chemical calculation. The anticancer effect of the compounds were explored in human non-small-cell lung cancer (NSCLC) A549 cell lines. As the results, the compound 3 and 4 induces toxicity and has proliferation inhibitory effects on A549 cells (IC50=58.99 mu M and 58.77 mu M, respectively) in vitro.