Watt-level all polarization-maintaining femtosecond fiber laser source at 1100 nm for multicolor two-photon fluorescence excitation of fluorescent proteins
arxiv(2024)
摘要
We demonstrate a compact watt-level all polarization-maintaining (PM)
femtosecond fiber laser source at 1100 nm. The fiber laser source is seeded by
an all PM fiber mode-locked laser employing a nonlinear amplifying loop mirror.
The seed laser can generate stable pulses at a fundamental repetition rate of
40.71 MHz with a signal-to-noise rate of >100 dB and an integrated relative
intensity noise of only 0.061
pulse compression, an output power of 1.47 W (corresponding to a pulse energy
of 36.1 nJ) and a pulse duration of 251 fs are obtained. The 1100 nm
femtosecond fiber laser is then employed as the excitation light source for
multicolor multi-photon fluorescence microscopy of Chinese hamster ovary (CHO)
cells stably expressing red fluorescent proteins.
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