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Inactivation of Oxidative Enzymes Using UV-C LEDs and Sensory Quality Assessment Using E-Nose and E-Tongue in Tender Coconut Water

Raj Kumar Maguluri, Akshay H. Dasalkar,Sudheer Kumar Yannam

Food and Bioprocess Technology(2024)

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Abstract
This study explores the impact of 280 nm ultraviolet-C light emission diodes (UV-C LEDs) on (polyphenol oxidase (PPO) and peroxidase (POD) enzymes in tender coconut water (TCW), addressing browning and flavour changes. Tender coconut water was treated with four doses (1, 2, 3, and 4 J/cm 2 ). At the highest dose (4 J/cm 2 ), both enzyme activities decreased, with PPO reduction (83%) exceeding POD (77%). No significant changes occurred in pH (4.88 ± 0.01), titratable acidity (0.067 ± 0.0), Brix (5.97 ± 0.12), minerals, and sugars at 4 J/cm 2 . Although L * and a * values remained consistent, a shift towards b * (0.15 ± 0.01 to 0.37 ± 0.02) occurred. Total phenolics and ascorbic acid decreased by 49.32% and 21.24%, respectively. E-Nose analysis showed no significant aroma changes, except a propanol peak in treated samples. E-Tongue revealed taste profile alterations, but multivariate data processing found no significant differences among treated TCW samples. This study suggests that environmentally sustainable 280 nm UV-C LEDs have the potential to control enzyme activity in TCW without adversely affecting key physico-chemical attributes.
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Key words
UV-C LEDs 280 nm,Polyphenol oxidase (PPO) and peroxidase (POD),Tender coconut water,E-Nose and E-Tongue
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