Full field-of-view hexagonal lattice structured illumination microscopy based on the phase shift of electro-optic modulators
OPTICS EXPRESS(2024)
摘要
High throughput has become an important research direction in the field of superresolution (SR) microscopy, especially in improving the capability of dynamic observations. In this study, we present a hexagonal lattice structured illumination microscopy (hexSIM) system characterized by a large field of view (FOV), rapid imaging speed, and high power efficiency. Our approach employs spatial light interference to generate a two-dimensional hexagonal SIM pattern, and utilizes electro-optical modulators for high-speed phase shifting. This design enables the achievement of a 210-mu m diameter SIM illumination FOV when using a 100x/1.49 objective lens, capturing 2048 x 2048 pixel images at an impressive 98 frames per second (fps) single frame rate. Notably, this method attains a near 100% full field -of -view and power efficiency, with the speed limited only by the camera's capabilities. Our hexSIM demonstrates a substantial 1.73 -fold improvement in spatial resolution and necessitates only seven phase -shift images, thus enhancing the imaging speed compared to conventional 2D-SIM. (c) 2024 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement
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